Transport behavior of selected nanoparticles with different surface coatings in granular porous media coated with Pseudomonas aeruginosa biofilm

Well-controlled laboratory column experiments were conducted to understand the influence of Pseudomonas aeruginosa (P. aeruginosa) biofilms on the transport of selected engineered nanoparticles (ENPs) in granular porous media representative of groundwater aquifers or riverbank filtration settings. To understand the importance of particle size on retention in the biofilm-coated granular (quartz sand) matrix, column experiments were carried out using nanosized (20 nm) and micrometer-sized (1 μm) sulfate-functionalized polystyrene latex particles (designated as 20 nSL and 1 mSL, respectively). Additional experiments conducted with nanosized (20 nm) carboxyl-modified latex particles (20nCL) and carboxyl-modified CdSe/ZnS quantum dots (QDs) provide information on the influence of particle surface chemistry on retention. Biofilm grown on the surface of the sand was characterized by total biomass quantification, confocal laser scanning microscopy (CLSM), and electrokinetic analysis. All four particles exhibit increased retention in the biofilm-coated packed bed: e.g., the attachment efficiency (α) of the 1 mSL particle increases from 0.40 to 1.7, whereas α for the 20 nSL particle increases from 0.04 to 0.10 in the biofilm-coated system. Particle surface chemistry can also influence the affinity of the ENPs for the biofilm coating as revealed by the greater attachment of the 20 nSL particle onto the biofilm-coated sand (α = 0.10) than its carboxylated counterpart (α = 0.04). Column experiments conducted using sand coated with growth medium (LB) or extracellular polymeric substances (EPS) extracted from P. aeruginosa biofilms further reveal that particle surface chemistry influences the interaction between the different ENPs and these coated sand surfaces. Namely, coating of sand surfaces with LB medium or bacterial EPS does not affect the transport of the sulfonated nanoparticle, but the LB coating leads to decreased retention of the carboxylated latex nanoparticle. Furthermore, our results show that EPS coatings are not necessarily good surrogates for biofilm-coated sand. Electrokinetic characterization of the clean and coated sand surfaces also reveals that the extent of particle retention is not controlled by electrical double layer interactions. Future studies should thus be aimed at improving our understanding of the fundamental mechanisms (both colloidal and noncolloidal) governing nanoparticle transport and fate in biofilm-laden granular aquatic environments.