|
|||||
|
|
| 一株产酯化酶菌株的分离鉴定及产酶 | |
| 引用本文： | 王晓丹,李付丽,胥思霞,班世栋,王荣村,沈锡翠,吴鑫颖,邱树毅. 一株产酯化酶菌株的分离鉴定及产酶[J]. 酿酒, 2014, 41(5): 42-47 |
| 作者姓名： | 王晓丹 李付丽 胥思霞 班世栋 王荣村 沈锡翠 吴鑫颖 邱树毅 |
| 作者单位： | 1. 贵州省发酵工程与生物制药重点实验室,贵州,贵阳550025;贵州大学生命科学学院,贵州贵阳550025 2. 贵州省发酵工程与生物制药重点实验室,贵州,贵阳550025;贵州大学酿酒与食品工程学院,贵州,贵阳,550025 3. 贵州青酒集团有限责任公司,贵州镇远,557702 |
| 基金项目： | 黔科合重大专项[2010]6004号，科技部科技支撑计划项目课题 |
| 摘 要： | 从浓香大曲中分离筛选得到一株产酯化酶的菌株,鉴定并研究了其产酯化酶的特性。利用微生物分离技术筛选得到产酯化酶的红曲霉FBKL3.0018,根据形态特征、培养特征、生理生化特征并结合分子生物学方法进行鉴定。结果鉴定菌株FBKL3.0018为红曲属的紫色红曲霉(Monascuspurpureus)。菌株的酯化力较高,可用于生产酯化酶制剂和高酯化大曲等。 |
| 关 键 词： | 浓香大曲 酯化酶 鉴定 红曲霉 |
Identification and Enzyme Production of the Strain Producing Esterifying Enzyme |
|
| Affiliation: | WANG Xiao-dan, LI Fu-li,XU Si-xia, BAN Shi-dong,WANG Rong-cun,SHEN Xi-cui, WU Xin-ying,QIU Shu-yi(1.Guizhou Provincial Key Laboratory of Fermentation Engineering and Biological Pharmacy,Guiyang, Guizhou,550025,China; 2.Guizhou Green liquor Co.,Ltd, Zhenyuan, Guizhou,557702,China; 3.College of LifeSciences, Guizhou University, Guiyang ,Guizhou,550025,China; 4.School of Liquor-making and Food Engineering, Guizhou University,Guiyang,Guizhou,550025,China) |
| Abstract: | The strain producing esterifying enzyme was isolated from luzhou-flavor daqu,and identified and researched it＇s characteristic of producing esterifying enzyme.We screened a strain monascus FBKL3.0018 by microorganism separate techniques,and combined with morphological characters,cultural characteristics,physiological and biochemical characteristics and molecular methods to identify it.The strain monasens FBKL3.0018 was identified as Monascuspurpureus.The strain is a high esterifying ability,it can be applied to produce esterifying enzyme preparation and Daqn high esterifying ability. |
| Keywords: | luzhou-flavor daqu esterifying enzyme identification monascus |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |