一种用于评价洗发水去屑功效的体外生物模型

以马拉色菌作为指示菌,建立了一种以猪皮为载体的生物模型。该生物模型可以用于评价头发护理产品的去屑功效。包含以下步骤:载体前处理;指示菌的接种;用洗发水处理猪皮模型,采用平板接触法进行抑菌效果测试;计算平板上马拉色菌的生长面积与平板面积之比作为有效指数P;以有效指数P评价产品的去屑功效。去屑剂评价结果表明,在0.1%~2.0%范围内,抑菌整体作用排序为BPTZPTOCT。洗发水评价结果表明:1种洗发水无去屑功效;3种洗发水有去屑功效;2种洗发水有较强去屑功效。

An in vitro model for evaluation of the anti-dandruff performance of hair care products

An in vitro model for evaluation of the anti-dandruff performance of hair care products was established. In this model, Malassezia was used as indicator bacteria with pig skin as substrate. This model includes the following steps: pretreatment of the substrate; inoculation of indicator bacteria; measurement of the bacteriostatic efficacy by plate contact method; calculation of the effective index P; evaluation of the anti-dandruff performance by the effective index P. Three kinds of anti-dandruff agent, BPT, ZPT and OCT were tested with the model. Results show that the order for antibacterial efficacy of the anti-dandruff agents with concentration range of 0.1%~2.0% is BPT>ZPT>OCT. Evaluation results of hair shampoo from commercial market show that: one displays no anti-dandruff efficacy; three displays medium anti-dandruff efficacy; two displays strong anti-dandruff efficacy.