天然椰子油提取物对H2O2诱导的HSF细胞氧化损伤的保护作用

采用倒置生物显微镜观察人皮肤成纤维细胞(HSF)的形态,通过MTT法检测细胞存活率,并通过检测细胞内活性氧(ROS)水平、总抗氧化能力和抗氧化酶活性探讨天然椰子油提取物对H_2O_2诱导的HSF细胞氧化损伤的保护作用。结果表明,天然椰子油提取物在质量浓度为12.5~50 mg/L范围内对H_2O_2诱导的HSF细胞氧化损伤具有保护作用,且呈浓度依赖性。天然椰子油提取物可以抑制H_2O_2引起的细胞形态损伤,提高细胞存活率,还可以通过降低细胞内ROS水平、提高细胞总抗氧化能力以及提高细胞内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性,进而起到保护HSF细胞氧化应激损伤的作用。

Protective effects of virgin coconut oil extracts on oxidative damage of human skin fibroblasts cells induced by H2O2

The protective effect of virgin coconut oil extracts on H2O2 induced oxidative damage of human skin fibroblasts (HSF) cells were studied. The HSF cell morphology is observed by inverted biological microscope, the cell viability is detected by MTT method, and the ROS level, the intracellular total antioxidation capacity and the activity of antioxidation enzyme (SOD, CAT and GSH-Px) are examined for investigating the effects of virgin coconut oil extracts on oxidative damage in HSF cells. The results show that the virgin coconut oil extracts could protect the HSF oxidative damage induced by H2O2 at concentration range of 12.5 to 50 mg/L with a concentration dependent manner. Virgin coconut oil extracts could inhibit the cell morphological damage induced by H2O2 and improve the survival rate of the cells. In addition, virgin coconut oil extracts could protect HSF cells from oxidative damage induced by oxidative stress through decreasing the intracellular ROS level, increasing intracellular total antioxidant capacity and the enzyme activity of SOD, CAT and GSH-Px.