海蜇、牛骨和鳕鱼皮胶原肽的透皮吸收性能研究

以海蜇、牛骨和鳕鱼皮胶原为原料,采用酶解制备了不同分子量(1 kDa和1~3 kDa)的6种胶原肽。利用透皮扩散试验仪,考察了时间、分子量及质量浓度对胶原肽透皮吸收性的影响,通过荧光显微镜观察了异硫氰酸荧光素(FITC)标记的胶原肽对小鼠皮肤的透皮吸收过程。结果表明,6种胶原肽的质量浓度均为10 g/L时,其在24 h时透皮吸收能力由大到小依次为:1 kDa鳕鱼皮胶原肽、1 kDa牛骨胶原肽、1 kDa海蜇胶原肽、1~3 kDa海蜇胶原肽、1~3kDa牛骨胶原肽和1~3 kDa鳕鱼皮胶原肽;1 kDa胶原肽透皮吸收能力优于1~3 kDa胶原肽。牛骨和海蜇胶原肽的质量浓度越大,透皮吸收性越好,而鳕鱼皮胶原肽则相反。3种来源胶原肽单位面积累积透过量均随时间延长而增加,分子量越大透皮吸收性能越差。

Investigation of transdermal absorption performance of collagen peptides from jellyfish, bovine bone and cod skin

Six kinds of collagen peptide with different molecular weights (<1 kDa and 1~3 kDa) were prepared separately by enzymolysis method with collagen obtained from jellyfish, bovine bone and cod skin as starting materials. Effects of time, molecular weight and mass concentration on the transdermal absorption performance of the peptides were examined by transdermal diffusion testing apparatus. Permeation processes of FITC-labeled collagen peptides through mouse skin were observed by fluorescence microscopy. Results showed that, when mass concentration of the six kinds of collagen peptide is 10 g/L, the order (from big to small) of capacity of transdermal absorption at 24 h is as follows: <1 kDa cod skin collagen peptide, <1 kDa bovine bone collagen peptide, <1 kDa jellyfish collagen peptide, 1~3 kDa jellyfish collagen peptide, 1~3 kDa bovine bone collagen peptide and 1~3 kDa cod skin collagen peptide. The transdermal absorption capacity of <1 kDa collagen peptide is better than that of 1~3 kDa collagen peptide. For collagen peptide from bovine bone and jellyfish, the increase of mass concentration will result in enhancing transdermal absorptivity; while for cod skin collagen peptide, the situation is on the contrary. For all the three kinds of source of collagen, the cumulative penetration capacity of collagen peptides per unit area increases with time, while decreases with molecular weight.