| C谱紫外线对体外培养小鼠胚细胞的影响 |
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| 引用本文： | 王冰 藤田和子. C谱紫外线对体外培养小鼠胚细胞的影响[J]. 辐射防护, 1997, 17(3): 200-207 |
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| 作者姓名： | 王冰 藤田和子 |
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| 作者单位： | 卫生部工业卫生实验所，东京大学理学部生物科学室，东邦大学医学部生物室 |
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| 摘 要： | 本实验对紫外线致体外培养的小鼠胚细胞的效应进行了研究。胚细胞取自１１天胎龄绵小鼠前，后肢芽及中脑，并用小脑成纤维母细胞株３Ｔ３进行参照。实验结果表明，在０－３０Ｊ／ｍ＾２的紫外线照射下观察到胚细胞生长和分化抑制作用及胸腺嘧啶聚合物和光产物的形成。紫外线对胚细胞分化的抑制作用比对增殖和抑制作用强，其对ＤＮＡ的损伤作用在胚细胞与参照细胞间相似，这两类细胞对ＤＮＡ损伤的修复动力学相似。
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| 关 键 词： | 胚细胞 C谱 紫外线 致畸 DNA损伤 小鼠 |
EFFECTS OF UVC IRRADIATION ON CULTURED MOUSE EMBRYONIC CELLS |
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| Wang BingLaboratory of Industral Hygiene,MPH,Beijing,, Kazuko Fujita. EFFECTS OF UVC IRRADIATION ON CULTURED MOUSE EMBRYONIC CELLS[J]. Radiation Protection, 1997, 17(3): 200-207 |
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| Authors: | Wang BingLaboratory of Industral Hygiene MPH Beijing Kazuko Fujita |
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| Affiliation: | Wang BingLaboratory of Industral Hygiene,MPH,Beijing,100088, Kazuko FujitaDepartment of Biology and Radioisotope Laborytory,Toho University School of Medicine,Tokyo 143,Japan, Takeshi Yamada ** Nobuhiro UchidaLaboratory of Radiation Bi |
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| Abstract: | proliferation and differentiation, dose dependent induction of DNA cyclobutane pyrimidine dimers and (6 4)photoproducts were found in the embryonic cells. Mesenchymal chomdrogenesis was more sensitive to the UVC than proliferation, and the UVC induced DNA damage and their repair kinetics in the cultured embryonic cells were similar to those in mouse 3T3 cells. No effects of treatments by the fluorescent light pre or post UVC irradiation were found on the repair kinetics of DNA damage in all of the cells. ( |
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| Keywords: | Embryonic Cell UVC Teratogenesis DNA Damage DNA Repair Mouse) |
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