Effect of pesticides on the methylene blue reduction test in milk

The effect of various pesticides on the methylene blue reduction test of milk was studied. Phosphamidon (0-(2-Chloro-2(diethylcarbamoyl)-1-methyl vinyl)-0-0-dimethyl phosphate), DDVP (0:0-dimethyl-2:2-dichlorovinyl phosphate), dimetilan (2-dimethyl carbamoyl-3-methyl pyrazolyl-(5)-dimethyl carbamate), 1-naphthol, NaDDC (sodium diethyl-dithiocarbamate), baygon (2-isopropoxyphenyl-N-methyl carbamate), isolan (1-isopropyl-3-methylprazolyl-(5)-dimethyl carbamate), pyramat (2-propyl pyrimidyl-(6)-dimethyl carbamate), and amidithion (0,0-dimethyl-S-(2-methoxyethyl carbamoyl-methyl) phosphorodithioate) in raw milk did not affect the reduction time compared to the control. Sevin (1-naphthyl-N-methyl carbamate) and pyrolan (1-phenyl-3-methyl pyrazolyl-(5)-dimethyl carbamate) in milk increased the reduction time slightly but not significantly. Thiram (Tetramethylthiuram disulphide, TMTD) increased the reduction time at .05 μg/ml or above. But this pesticide did not significantly affect bacterial growth in milk.