黏着斑激酶生物信息学分析

从生物信息学的角度对黏着斑激酶进行分析,为进一步研究其高级结构及探寻黏着斑激酶的潜在功能提供理论数据.用生物信息学方法对已在GenBank上注册的人、牛、鸡、非洲蟾蜍、斑马鱼等动物黏着斑激酶基因的核苷酸序列以及推导的氨基酸序列、组成成分、氨基酸翻译后修饰、信号肽、跨膜拓朴结构域、疏水性/亲水性、蛋白质二级结构以及三级结构等进行分析预测和推断.结果表明:这些动物的黏着斑激酶属于非跨膜的亲水性蛋白,α-螺旋和不规则卷曲是其蛋白质二级结构的主要结构元件,β-转角和延伸链散布在整个蛋白质中,包含三个结构域,即N末端FERM同源结构域,中部的激酶结构域和C末端富含脯氨酸的位点和FAT结构域.

Bioinformatics Analysis of Focal Adhesion Kinase in Different Animals

In the present study,the nucleic acid sequence and amino acid sequence of focal adhesion kinase from human,cattle,chicken,mouse,African clawed frog,zebrafish,green sea urchin and so on,which were registered in GenBank,were analyzed and predicted by the tools of bioinformatics in the following aspects: the composition of nuclei acid sequences and amino acid sequences,signal peptide,trans-membrane topological structure,hydrophobicity or hydrophilicity,secondary structure and tertiary structure,etc.The results were as follows: Focal adhesion was a hydrophilic and non-transmembrane protein without signal peptide in N-terminal;the main motifs of predicted secondary structure of FAK were alpha helix and random coil,beta turn and extended strand were spread to the whole secondary structure of protein;amino acid sequences of FAK include three conserved functional domains,the FERM homology domain in N-terminal,the catalytic domain in central domain and the FAT domain and sites for multiple protein-protein interactions in C-terminal.Consequently,a series of useful analyses for focal adhesion kinase were gained by bioinformatics.