反式茴脑生物降解菌株的筛选及其生物转化

以广西特色香料资源茴油为碳源，开展了反式茴脑生物降解菌株的筛选工作，并对反式茴脑生物转化过程进行了初步研究，以期获得的菌株能催化茴油中的主要成分反式茴脑，合成高附加值的天然香料或医药中间体。通过富集培养的方法，从八角树下土壤中筛选能将反式茴脑转化生成茴香醛或茴香酸的细菌菌株，采用薄层层析法、分光光度法和HPLC法相结合对转化液进行分析。结果筛选到一株细菌菌株BT-13能以茴油为碳源高产茴香酸。从形态、生理生化实验和16S rDNA序列分析，初步鉴定细菌菌株BT-13为假单胞菌属（Pseudomonas sp.）。

Isolation of trans-anethole degrading bacterial strains and biotransformation of trans-anethole

Anise oil is a natural spice in Guangxi. Isolation of trans-anethole degrading bacterial strains and trans-anethole biotransformation process were performed using anise oil as the only carbon source. The target strains were expected to produce high value-added nature spice and medicine intermediate by biotransformation of trans-anethole—the main constituent of anise oil. Bacteria were isolated from soil under aniseed trees by enrichment culture. Then biotransformation of trans-anethole to anisaldehyde or anisic acid with these strains were carried out by fermentation. The contents of trans-anethole，anisaldehyde and anisic acid in the culture broth were analyzed by the methods of TLC，photometry and HPLC determined. The isolated bacterial strain BT-13 had good capability to biotransform trans-anethole to anisic acid. This strain was identified as Pseudomonas sp. according to visual observation，physiological and biochemical experiments and 16S rDNA sequence analysis.