Separation and Quantification of Vegetable Oil Based Polyols by High Performance Liquid Chromatography with Evaporative Light Scattering Detection |
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Authors: | Jin Yue Suresh S Narine |
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Affiliation: | (1) Alberta Lipid Utilization Program, Department of Agricultural Food and Nutritional Science, University of Alberta, 4-10 Agriculture/Forestry Centre, Edmonton, AB, Canada, T6G 2P5 |
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Abstract: | Polyols with terminal primary alcohol functionalities were obtained from canola oil via an ozonolysis and hydrogenation process.
A high performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for
separating and quantifying the polyol products. Linear calibration curves were obtained for the mono-ol, diol and triol components
with correlations (r
2) above 0.98. According to the standard curve, the content of mono-ol, diol and triol can be obtained from their HPLC-ELSD
chromatograms. |
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Keywords: | Polyol Canola oil High performance liquid chromatography Evaporative light scattering detection Calibration curve |
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