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Separation and Quantification of Vegetable Oil Based Polyols by High Performance Liquid Chromatography with Evaporative Light Scattering Detection
Authors:Jin Yue  Suresh S Narine
Affiliation:(1) Alberta Lipid Utilization Program, Department of Agricultural Food and Nutritional Science, University of Alberta, 4-10 Agriculture/Forestry Centre, Edmonton, AB, Canada, T6G 2P5
Abstract:Polyols with terminal primary alcohol functionalities were obtained from canola oil via an ozonolysis and hydrogenation process. A high performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for separating and quantifying the polyol products. Linear calibration curves were obtained for the mono-ol, diol and triol components with correlations (r 2) above 0.98. According to the standard curve, the content of mono-ol, diol and triol can be obtained from their HPLC-ELSD chromatograms.
Keywords:Polyol  Canola oil  High performance liquid chromatography  Evaporative light scattering detection  Calibration curve
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