| 响应面法优化芽孢杆菌发酵生产植酸酶 |
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| 引用本文： | 于平,陈益润. 响应面法优化芽孢杆菌发酵生产植酸酶[J]. 中国粮油学报, 2011, 26(3): 86-90 |
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| 作者姓名： | 于平 陈益润 |
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| 作者单位： | 浙江工商大学食品与生物工程学院,杭州,310035 |
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| 基金项目： | 浙江省教育厅重点研究项目 |
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| 摘 要： | 对实验室自行分离得到的芽孢杆菌ZJ0702发酵生产植酸酶的条件进行优化,以提高植酸酶的产量。利用2-level Factorial试验筛选出对产酶显著影响的3个因素,通过最陡爬坡试验确定中心点,再采用Central Composite设计对重要因子进行优化。结果发现麸皮、蛋白胨和KH2PO4的添加量是影响植酸酶产量的显著因素,经优化后的培养基组分为3.7%麸皮,2.27%蛋白胨,0.5%硝酸铵0,.008 63%无机磷0,.2%CaCl2,0.05%KCl,0.03%MgSO4,0.003%FeSO4,0.003%MnSO4,0.03%NaCl;培养条件为:34℃,接种量7%,pH7.0,摇瓶装液量75 mL/250 mL,在上述条件下该芽孢杆菌84 h产酶达到最高值13 625.8 U/mL,与优化前菌株产酶活力相比,酶活提高了19.6%。
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| 关 键 词： | 芽孢杆菌 植酸酶 优化 响应面法 |
Application of Response Surface Methodology to Optimize the Fermentation Conditions for Producing Phytase from Bacillus subtilis |
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| Yu Ping,Chen Yirun. Application of Response Surface Methodology to Optimize the Fermentation Conditions for Producing Phytase from Bacillus subtilis[J]. Journal of the Chinese Cereals and Oils Association, 2011, 26(3): 86-90 |
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| Authors: | Yu Ping Chen Yirun |
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| Affiliation: | Yu Ping Chen Yirun (College of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310035) |
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| Abstract: | The fermentation conditions for producing phytase by Bacillus subtilis ZJ0702 were optimized in order to enhance its activity.Three factors influencing phytase activity significantly were screened by 2-level factorial experiment,the central point was determined by the steepest ascent experiment and the optimal fermentation conditions were established by central composite design.The results indicated that wheat bran,tryptone and KH2PO4 were the three main factors which have an obvious effect on the phytase a... |
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| Keywords: | Bacillus subtilis phytase optimizing response surface |
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