Polyurethane/Polylactide-Based Electrospun Nonwovens as Carriers for Human Adipose-Derived Stromal Stem Cells and Chondrogenic Progenitor Cells |
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| Authors: | Krzysztof Marycz Monika Marędziak Jakub Grzesiak Ryszard Fryczkowski Jadwiga Laska |
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| Affiliation: | 1. Electron Microscopy Laboratory, Wroclaw University of Environmental and Life Sciences, Wroclaw, Lower Silesia, Polandkrzysztofmarycz@interia.pl;3. Department of Animal Physiology and Biostructure, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroc?aw, Lower Silesia, Poland;4. Department of Biotechnology, Wroclaw Research Center EIT+, Wroc?aw, Lower Silesia, Poland;5. Faculty of Materials and Environmental Sciences, ATH University of Bielsko-Biala, Bielsko-Biala, Silesia, Poland;6. Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Krakow, Lesser Poland, Poland |
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| Abstract: | Articular cartilage dysfunctions are major cause of pain and disability and lead to serious health complications. Cell-based therapies are proposed as treatment methods for cartilage regeneration. In this study, we proposed polyurethane/poly(L-lactide-co-D, L-lactide)-based electrospun nonwovens as carriers for the delivery of human adipose-derived stromal stem cells. We found that 6:4 and 8:2 polyurethane/poly(L-lactide-co-D, L-lactide) initially enhance proliferative rate of human adipose-derived stromal stem cells, shorten their population doubling time, promote creation of functional chondrogenic nodules during chondrogenic differentiation, improve the collagen-2-to-collagen-1 protein ratio, and upregulate the expression of collagen-2 and aggrecan genes. |
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| Keywords: | Chondrogenic progenitor cells electrospinning mesenchymal stem cells polylactide polyurethane |
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