“一步法”制备纳米相丝素蛋白/羟基磷灰石生物复合材料

系统分析了羟基磷灰石（HA）的制备方法和丝素蛋白纤维（SF）的溶解方法，提出一种制备纳米丝素蛋白/羟基磷灰石生物复合材料（SF/HA）的新型反应复合方法——“一步法”。并对由“一步法”制得的SF/HA分别进行了钙磷比测定、红外光谱测试、透射电镜观察和X射线衍射测试。结果表明：SF/HA中的钙磷比是1.6692，与标准HA中的钙磷比1.67一致；SF/HA中同时含有SF和HA中各自的官能团；SF/HA的晶粒横向尺度小于100 nm，SF/HA呈针状或柱状晶粒， SF和HA能够形成复合； SF/HA的晶型属于六方晶系， 当SF在SF/HA中所占质量分数为10%时，晶胞参数a=b=9.0319 A，c=7.0148 A，沿c轴方向平均晶粒尺寸是230.7645 A。“一步法”制备SF/HA具有合理性和可行性。

Preparation of nano silk fibroin/hydroxyapatite biological composite by “one-step&ldquo|method

On the basis of analyzing the preparation method of hydroxyapatite (HA) and the diffluence method of silk fibroin fibre (SF)，a  “one-step” method that is used to prepare nano hydroxyapatite/silk fibroin biology composite (SF/HA) was  put forward. The SF/HA composite was observed and tested by FTIR, TEM and XRD.The ratio of calcium to phosphorus in the SF/HA composite was tested by the chemical method.The results show that the ratio of calcium to phosphorus in the SF/HA composite is 1.6692, which  is the same as 1.67 in HA.The function groups in both HA and SF were discovered in the SF/HA composite. The SF/HA composite crystal particle is of spiculate or column configuration. The crystal size of the SF/HA composite is less than 100 nm in the horizontal direction. The HA and SF composite can come into being. The HA/SF composite is hexahedron crystal.When the mass fraction  of SF in SF/HA was 10%，the crystal cell parameter of the SF/HA composite crystal is a=b=90319 A，c=7.0148 A. The average crystal size of SF/HA along the c axes direction is 230.7645 A. So the “one-step” method is rational and feasible.