Parallel flow measurements in microstructures by use of a multifocal 4 x 1 diffractive optical fan-out element |
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Authors: | Blom Hans Johansson Mathias Gösch Michael Sigmundsson Toni Holm Johan Hård Sverker Rigler Rudolf |
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Affiliation: | Department of Microelectronics and Information Technology, Royal Institute of Technology, Kista, Sweden. blom@ele.kth.se |
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Abstract: | We have developed a multifocal optical fluorescence correlation spectroscopy system for parallel flow analyses. Multifocal excitation was made possible through a 4 x 1 diffractive optical fan-out element, which produces uniform intensity in all four foci. Autocorrelation flow analyses inside a 20 microm x 20 microm square microchannel, with the 4 x 1 fan-out foci perpendicular to the flow direction, made it possible to monitor different flows in all four foci simultaneously. We were able to perform cross-correlation flow analyses by turning the microstructure, thereby having all four foci parallel to the direction of flow. Transport effects of the diffusion as a function of flow and distance could then also be studied. |
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