Detection of 4-aminocarminic acid in beverages and cake glaze from the German market: a food additive not approved in the EU |
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Authors: | Andreas Miller Eva Lassek Julia Pfister Richard Klinger Werner Höbel Holger Knapp Franziska Gaßmann Uwe Wollein Nicholas Schramek |
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Affiliation: | 1.The Bavarian Health and Food Safety Authority,Oberschlei?heim,Germany;2.The Bavarian Health and Food Safety Authority,Würzburg,Germany;3.The Bavarian Health and Food Safety Authority,Erlangen,Germany |
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Abstract: | In the European Union, carminic acid and its ammonium, calcium, potassium or sodium salts and its aluminium lakes are approved as food additive E120. In beverages obtained from the German market 4-aminocarminic acid (“acid-stable carmine”) was detected by HPLC–PDA and LC–MS/MS. Isolation of the colorant from a liquid dye preparation used for the production of a beverage sample and subsequent NMR analysis confirmed the presence of 4-aminocarminic acid. Synthesis of 15N-4-aminocarminic acid clearly demonstrated that “acid-stable carmine” is not the ammonium salt of carminic acid, which is approved as food additive in the European Union. In fact, nitrogen in “acid-stable carmine” is covalently bound. The molecular structure of carminic acid is chemically modified and 4-aminocarminic acid does not comply with the specifications laid down for E120 in Commission Regulation (EU) No 231/2012. 4-Aminocarminic acid was also detected in the red-colored glaze of a raspberry cake and in the liquid dye preparation used for coloring this glaze. |
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