非定向筛查数据非依赖采集策略研究进展

数据采集方式是质谱分析的关键步骤,是影响实验效率以及结果准确度的重要因素。较之于传统的数据采集方式,近年来出现的数据非依赖采集技术具有高通量、高覆盖度、高准确性等优势,从而成为蛋白组学与代谢产物定量和组学研究的强有力工具,对食品分析领域有重要的参考和借鉴价值。综述了目前主要的几种数据非依赖采集原理和数据处理最新进展并突出了在食品分析中的非定向筛查潜力,总结了非定向筛查数据非依赖采集在食品安全性分析、食品真实性分析,食品中功能活性成分分析的应用。最后探讨数据非依赖采集在食品分析中面临的挑战和未来发展趋势。

Advance in non-targeted screening for data independent acquisition

Data acquisition is an essential poeedure affecting the efficieney and accuracy of the experiment in mass spectrometry analysis. Compared with the traditional data acquisition methods, the data independent acquisition technolggy emerging in recent yearshas the advantages of high throughput,high eoverage and high aceuraey,which has hecome a powerful tool for proteomics, metabolite quantitative and omics researeh, and has important referenee value in the field of food analysis. The principles of the curent main datain dependent acquisition and recent advance in data processing were reviewed as well as the potential of non-targeted screening in food analysis was highlighted. Furthermore,the application of non-targeted screening data independent acquisition in analysis of food safety,food anthenticity and funetional active ingredients was summarized. Finally, the challenges and future trends of data independent acquisition in food analysis was discussed.