| 里氏木霉LW1产纤维素酶的酶学性质 |
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| 引用本文： | 王菁莎,王颉,刘景彬. 里氏木霉LW1产纤维素酶的酶学性质[J]. 纤维素科学与技术, 2006, 14(2): 46-49 |
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| 作者姓名： | 王菁莎 王颉 刘景彬 |
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| 作者单位： | 1. 河北农业大学,食品科技学院,河北,保定,071001
2. 天津科技大学食品科学与生物技术学院,天津,300222 |
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| 摘 要： | 研究了里氏木霉LW1所产纤维素酶的主要酶学性质。结果表明,该纤维素酶的最适温度为50℃,最适pH值为4.8;在30～50℃,pH 4.0～6.0范围内有较高的稳定性;90℃处理15 min,酶粉的CMC酶活和FPA酶活保存率分别为38.66%和52.68%;Ca2+、K+、Na+、Mg2+离子对酶活有激活作用,Mn2+、Zn2+、Cu2+离子有抑制作用。
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| 关 键 词： | 里氏木霉 纤维素酶 酶学性质 |
| 文章编号： | 1004-8405(2006)02-0046-04 |
| 修稿时间： | 2005-09-12 |
Enzymatic Characteristics of Cellulase Produced by Trichoderma Reesei LW1 |
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| WANG Jing-sha,WANG Jie,LIU Jing-bin. Enzymatic Characteristics of Cellulase Produced by Trichoderma Reesei LW1[J]. Journal of Cellulose Science and Technology, 2006, 14(2): 46-49 |
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| Authors: | WANG Jing-sha WANG Jie LIU Jing-bin |
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| Abstract: | Enzymatic characteristics of the crude enzyme from Trichoderma Reesei LW1 were studied.The optimal conditions for enzymatic reaction were 50℃,and pH value were 4.8;showed a high stability at temperature 30～50℃,pH 4.0～6.0;stayed 15 min at 90℃,CMCase could be held 38.66%,and FPase can be held 52.68%;Ca2+、K+、Na+、Mg2+ were the activators and Mn2+、 Zn2+、Cu2+ were the inhibitors of the enzyme. |
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| Keywords: | Trichoderma Reesei cellulase enzymatic characteristics |
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