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Removal of Phytate from Great Northern Beans (Phaseolus vulgaris L.) and Its Combined Density Fraction
Authors:N R REDDY  S K SATHE  M D PIERSON
Affiliation:Authors Reddy and Pierson are with the Dept. of Food Science &Technology, Virginia Polytechnic Institute &State Univ., Blacksburg, VA 24061. Author Sathe is with the Dept. of Foods &Nutrition, Purdue Univ., West Lafayette, IN 47907.
Abstract:The effect of pH on the dissociation of phytate complexes and subsequent removal of phytate from Great Northern bean (GNB) flour and its combined density fraction (CDF) was investigated. The largest amount of phytate removed was from GNB flour and CDF dispersions adjusted to pH 7.0 during dialysis. Dialyzed, unadjusted GNB flour and CDF respectively contained 55.4% and 59% of phytate in water-soluble form. The results indicate that phytic acid is present in GNB as a water soluble salt with a M.W. less than 1,000 daltons, water soluble complex with M.W. more than 1,000 daltons, and water insoluble complex. The presence of phytate in the CDF did not affect in vitro digestibility of CDF proteins.
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