复合酶法提取蛹虫草类胡萝卜素工艺优化及其抗氧化能力测定

为确定蛹虫草类胡萝卜素的最佳提取工艺条件，通过单因素实验和响应面实验设计分析探究复合酶法提取蛹虫草类胡萝卜素的最佳工艺条件并采用紫外光谱法测定其体外抗氧化活性并与传统酸热提取法下得到类胡萝卜素的抗氧化能力进行比较。结果显示:纤维素酶∶果胶酶（2∶1）时，酶解温度50 ℃、酶解时间45 min、pH=4、酶浓度为0.5%，蛹虫草类胡萝卜素的提取率可达3 796.23 μg/g，与传统酸热提取法相比提取率得到显著提高。体外抗氧化活性测定结果显示，复合酶法提取得到的类胡萝卜素的总抗氧化能力为4.151 U/mg，酸热提取法下得到的类胡萝卜素总抗氧化能力为4.096 U/mg。即2种提取方法下获得的蛹虫草类胡萝卜素的体外抗氧化活性相差不大。

Extraction and Anti-oxidant Activity of Carotenoids from Cordyceps Militaris by Multi-enzyme Method

In order to determine the optimum conditions of cordyceps militaris carotenoids, single factor experiment and response surface experiment were carried out to explore the complex enzymatic method optimum process conditions of cordyceps militaris carotenoids. Ultraviolet spectrum method was used to measure its antioxidant activity in vitro. The acitivity was compared with the antioxidant activity obtained by the traditional acid heat extraction.The results show that under the conditions of cellulase∶pectinase (2∶1), the enzymolysis time is 45 min、enzymolysis temperature 50 ℃、pH=4、the concentration of enzymes 0.5%. The extraction rate of Carotenoids from Cordyceps militaris is up to 3 796.23 μg/g under the optimum extraction parameters, which gets a big improvement compared with acid-heating method. The in vitro antioxidant activity assay shows that the total antioxidant capacity of carotenoids obtained by the complex enzymatic method is 4.151 U/mg, and that of carotenoids obtained by the acid heat extraction method is 4.096 U/mg. In other words, the antioxidant activities of cordyceps militaris carotenoids obtained by the two extraction methods were not significantly different in vitro.