酯化型红曲菌复合诱变选育及其固态发酵条件优化

为了获得高酯化力红曲菌，采用紫外线结合常压室温等离子体复合诱变对实验室保藏的红曲菌进行诱变育种，并通过单因素实验及响应面分析法优化其固态发酵条件，提高菌株产酯化酶能力。出发菌株N3经过复合诱变，采用透明圈法初筛及酯化酶活力测定法复筛，得到1株产酯化酶活力高且遗传稳定性较好的诱变菌株Z14，其酶活稳定至34.44 U/g，较出发菌株提高了74%。通过响应面分析法得到优化的固态发酵条件为:接种量为5%、含水量为70%、培养时间为7 d。在该条件下Z14产酯化酶活力达到38.53 U/g，较出发菌株提高了95%。

Breeding of Esterified Monascus Compound Mutagenesis and Optimization of Solid State Fermentation Conditions

In order to obtain high-esterification Monascus,laboratory-prepared Monascus strains were mutated using UV combined with atmospheric pressure room temperature plasma compound mutagenesis,and their solid-state fermentation conditions were optimized through single-factor tests and response surface analysis.Improve the ability of strains to produce esterase.The starting strain N3 was subjected to compound mutagenesis,and the transparent circle method was used for preliminary screening and the esterification enzyme activity determination method was re-screened to obtain a mutant strain Z14 with high esterification enzyme activity and good genetic stability.The enzyme activity was stable to 34.44 U/g,which was 74%higher than the starting strain.The optimized solid-state fermentation conditions obtained by response surface analysis were:Inoculation amount was 5%,water content was 70%,and culture time was 7 days.Under these conditions,the esterase activity of Z14 reached 38.53 U/g,which was 95%higher than the starting strain.