Optimization of multi-stage countercurrent extraction of antioxidants from Ginkgo biloba L. leaves |
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Authors: | Chen-huan Yu Jing Chen Yao-kang Xiong Xiao-xian Li Xiao-yan Dai Chang-cheng Shi |
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Affiliation: | 1. Zhejiang Academy of Medical Sciences, Hangzhou 310013, China;2. College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China;3. Nanjing University of Chinese Medicine, Nanjing 210046, China |
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Abstract: | Multi-stage countercurrent extraction (MCE) as a novel extraction technique was used to extract antioxidants from Ginkgo biloba leaves. Orthogonal array design (OAD) was employed to optimize the ratio of 60% ethanol to raw material (8–16 mL/g), extraction time (30–60 min) and extraction temperature (60–80 °C) to obtain a high yield of antioxidants from G. biloba leaves by MCE. The optimum conditions were a ratio of 60% ethanol to raw material of 16 mL/g and extraction time of 30 min at 80 °C. Under these conditions, the yields of flavonoids and total phenolics were 1.74% and 2.42%, respectively, and DPPH radicals scavenging activity of the extract was 89.97%. Compared with heat-reflux extraction, MCE had obvious advantages of less extraction time and lower solvent and energy consumption. It may be used as a promising technique for the extraction of bioactive compounds from plant materials. |
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