| 重组大肠杆菌产胆固醇氧化酶的指数流加策略 |
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| 引用本文： | 孙艳,王长城,张玲,杨海麟,王武.重组大肠杆菌产胆固醇氧化酶的指数流加策略[J].化工进展,2010,29(1). |
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| 作者姓名： | 孙艳 王长城 张玲 杨海麟 王武 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室,江苏,无锡,214122 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 以指数流加策略高密度培养重组大肠杆菌,发酵生产胆固醇氧化酶。通过对比生长速率的分阶段控制使得细胞干重达40.128g/L。确定了最佳的诱导时机为菌体对数生长期的中期,产酶速率为1287.31U/(L·h)。菌体产酶水平达6436.56U/L,生产强度为459.75U/(L·h),实现了胆固醇氧化酶的高效生产。
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| 关 键 词： | 胆固醇氧化酶 高密度培养 指数流加 诱导时机 |
Exponential fed-batch culture strategy for the production of cholesterol oxidase in recombinant E. coli JM109-COD |
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| SUN Yan,WANG Changcheng,ZHANG Ling,YANG Hailin,WANG Wu.Exponential fed-batch culture strategy for the production of cholesterol oxidase in recombinant E. coli JM109-COD[J].Chemical Industry and Engineering Progress,2010,29(1). |
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| Authors: | SUN Yan WANG Changcheng ZHANG Ling YANG Hailin WANG Wu |
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| Abstract: | Exponential fed-batch culture strategy were carried out to produce cholesterol oxidase (COD) using recombinant E. coli high cell-density culture. High cell-density was achieved by specific growth rate grading-control, and the final cell dry weight was 40.128g/L. The optimum induction time was determined as in the mid-logarithmic phase. Under the optimized cultivation conditions, the COD production rate was 1287.31U/(L·h). The highest COD activity (6436.56U/L) and highest COD productivity 459.75U/(L·h)] were achieved. |
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| Keywords: | cholesterol oxidase high cell-density culture exponential fed-batch induction time |
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