Isolation and characterization of ganglioside 9-O-acetyl-GD3 from bovine buttermilk |
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| Authors: | Daniela M. Bonafede Lawrence J. Macala Martha Costantine-Paton Robert K. Yu |
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| Affiliation: | (1) Department of Biology, Yale University, 06511 New Haven, CT;(2) Department of Neurology, Yale University Medical School, 06511 New Haven, CT;(3) Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Virginia Commonwealth University, 23298-0614 Richmond, VA |
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| Abstract: | Bovine buttermilk contains a unique ganglioside, 9-O-acetyl-GD3. In order to isolate large quantities of this ganglioside, a simplified isolation scheme which consists of several ion-exchange and silica gel column chromatographic procedures was devised. The isolated 9-O-acetyl-GD3 was characterized on the basis of its thin-layer chromatographic behavior, its immunoreactivity with a specific monoclonal antibody, JONES, and by conversion to authentic GD3 by mild base treatment. |
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