The effect of transient proanthocyanidins preconditioning on the cross-linking and mechanical properties of demineralized dentin |
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Authors: | Ruirui Liu Ming Fang Yuhong Xiao Fang Li Lan Yu Sanjun Zhao Lijuan Shen Jihua Chen |
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Affiliation: | (1) Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, #145 Changle Xi Road, 710032 Xi’an, People’s Republic of China;(2) Department of Stomatology, Kunming General Hospital of PLA, Kunming, China;(3) Department of Microbiology, Fourth Military Medical University, #145 Changle Xi Road, Xi’an, People’s Republic of China; |
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Abstract: | Proanthocyanidin-based preconditioners were prepared by adding powdered proanthocyanidins-rich grape seed extract to various
solvents at different concentrations. Demineralized dentin specimens were preconditioned for 20, 30, 60 or 120 s, followed
by the evaluation of their cross-linking degree, mechanical properties and micromorphology. The cross-linking degree of the
demineralized dentin collagen exhibited concentration- and time- dependent increase after preconditioning treatment, irrespective
of the preconditioner and the solvent. When treated for the same exposure time, specimens after 15% proanthocyanidins preconditioning
resulted in the highest mean ultimate tensile strength compared with all the other groups tested. Five percent glutaraldehyde
control group produced the highest cross-linking degree, but the ultimate tensile strength was lower than that of 15% proanthocyanidins
group. The field emission scanning electron microscopy confirmed that the demineralized dentin collagen was in a homogeneous
and regular arrangement after preconditioning and maintained expanding, regardless of the surface moisture conditions. |
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