Interference of polar lipids with the alkalimetric determination of free fatty acid in fish lipids |
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Authors: | Shengying Zhou Robert G Ackman |
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Affiliation: | (1) Canadian Institute of Fisheries Technology, Technical University of Nova Scotia, P.O. Box 1000, B3J 2X4 Halifax, Nova Scotia, Canada |
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Abstract: | An examination of the suitability of an alkalimetric method for the determination of free fatty acid (FFA) contents in fats,
oils, and lipid extracts was conducted by comparing AOCS method Ca 5a-40 with a method based on a Chromarod-latroscan thin-layer
chromatography-flame-ionization detector (TLC-FID) system. The FFA contents determined by the alkalimetric method were consistently
higher than the genuine FFA contents obtained by the latroscan TLC-FID method. Phospholipids were found to be the major components
that contributed to the alkali-titratable, nongenuine FFA in the total FFA determined alkalimetrically. Contributions from
other polar lipid components were smaller, but they dominated as the proportion of phospholipids fell. The other alkali-titratable
polar components may include oxidized lipids and their by-products bound to protein fragments. The accurate determination
of FFA contents by alkalimetric methods may only be applicable to those commercially refined fats and oils that contain negligible
amounts of phospholipids. Corrections for the alkalimetrically determined FFA contents should be made for those fats and oils
with relatively high phospholipid contents by correlating the nongenuine FFA contents and the phospholipid contents. |
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Keywords: | Alkalimetric titration free fatty acid free fatty acid content latroscan TLC-FID oils lipid class phospholipid polar lipid |
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