A Specific Groove Pattern Can Effectively Induce Osteoblast Differentiation |
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Authors: | Chang‐Su Kim Jin‐Hee Kim Bokyoung Kim Young‐Seok Park Hong‐Kyun Kim Hieu Trung Tran Sang Hoon Kim Hojeong Jeon Sangjib Kim Ji Hyun Sim Hyun Mu Shin Gwanghun Kim Young Joo Baik Kee‐Joon Lee Hae‐Young Kim Tae Jin Yun Youn Sang Kim Hang‐Rae Kim |
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Affiliation: | 1. Department of Anatomy and Cell Biology, Seoul National University College of Medicine, Seoul, Republic of Korea;2. Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea;3. Department of Biomedical Laboratory Science, College of Health Science, Cheongju University, Cheongju, Republic of Korea;4. Program in Nano Science and Technology, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Republic of Korea;5. Green Battery Innovation Research Center, Advanced Institutes of Convergence Technology, Gyeonggi‐do, Republic of Korea;6. Department of Oral Anatomy, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Republic of Korea;7. Materials Architecturing Research Center, Korea Institute of Science & Technology, Seoul, Republic of Korea;8. Center for Biomaterials, Korea Institute of Science & Technology, Seoul, Republic of Korea;9. Department of Mathematics, College of Science, Korea University, Seoul, Republic of Korea;10. BK21Plus Biomedical Science Project, Seoul National University College of Medicine, Seoul, Republic of Korea;11. Medical Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea;12. Department of Orthodontics, College of Dentistry, Yonsei University, Seoul, Republic of Korea;13. Department of Health Policy and Management, Collage of Health Sciences, Korea University, Seoul, Republic of Korea;14. Department of Radiology, Seoul National University Hospital, Seoul, Republic of Korea |
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Abstract: | Little is known about the principles of surface structure design for orthopedic and dental implants. To find topographical groove patterns that could enhance osteoblast differentiation according to cell type, groove patterns are fabricated with ridges (0.35?7 µm) and grooves (0.65?6 µm) of various widths and explored their mechanisms in improving osteoblast differentiation. This study finds that a groove pattern enhancing osteoblast differentiation is associated with the ability of the cell to extend its length and that it is able to overcome the inhibition of osteoblast differentiation that takes place under inflammatory conditions. The groove pattern suppresses the generation of reactive oxygen species, a reaction that is increased in inflammatory conditions. It also modulates the expression of osteogenic factors according to differentiation time. Importantly, specific groove patterns AZ‐2 and AZ‐4, with ridge width of 2 µm and groove width of 2 or 4 µm, respectively, effectively promote bone regeneration in critical‐sized calvarial defects without additional factors. This knowledge of groove patterns can be applied to the development of orthopedic and dental devices. |
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Keywords: | bone regeneration groove pattern inflammation osteoblast reactive oxygen species |
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