| 海洋放线菌JWH-09代谢产物中农用抗菌组分的分离纯化及结构鉴定 |
| |
| 引用本文： | 魏刚,薛陕,周瑜,宗志友,陶黎明.海洋放线菌JWH-09代谢产物中农用抗菌组分的分离纯化及结构鉴定[J].农药,2010,49(12). |
| |
| 作者姓名： | 魏刚 薛陕 周瑜 宗志友 陶黎明 |
| |
| 作者单位： | 华东理工大学上海市化学生物学(芳香杂环)重点实验室;上海师范大学生命与环境科学学院; |
| |
| 基金项目： | 国家高科技研究发展(863)计划，上海市优秀学科带头人计划 |
| |
| 摘 要： | 从134株海洋微生物中筛选得到放线菌JWH-09,其发酵代谢产物具有较好的抗植物病原真菌活性.根据生物活性跟踪测试,采用硅胶柱层析、薄层层析以及制备高效液相色谱等技术对JWH-09发酵产物中的抗菌活性组分进行分离纯化,得到了一种抗真菌活性化合物JWH-09-A,经ESI-MS、EI-MS、1H NMR、13C NMR、2D NMR等谱图分析,确定该活性物质的结构为1-菲羧,1,2,3,4,4a,9,10,10a-八氢-1,4a-二甲基-7-(1-甲基乙基),俗名脱氢松香酸(dehydroabietic acid).经盆栽试验证明该化合物对水稻纹枯病、黄瓜白粉病和灰霉病具有较好的防治效果.
|
| 关 键 词： | 海洋放线菌JWH-09 抗真菌活性 分离纯化 脱氢松香酸 |
Isolation, Purification and Identification of Antifungal Compound from the Metabolites of Marine Actinomycete JWH-09 |
| |
| WEI Gang,XUE Shan,ZHOU Yu,ZONG Zhi-you,TAO Li-ming.Isolation, Purification and Identification of Antifungal Compound from the Metabolites of Marine Actinomycete JWH-09[J].Pesticides,2010,49(12). |
| |
| Authors: | WEI Gang XUE Shan ZHOU Yu ZONG Zhi-you TAO Li-ming |
| |
| Affiliation: | WEI Gang1,XUE Shan2,ZHOU Yu1,ZONG Zhi-you2,TAO Li-ming1(1.Shanghai Key Lab of Chemical Biology,East China University of Science and Technology,Shanghai 200237,China,2.College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China) |
| |
| Abstract: | The metabolites of an actinomycete JWH-09 which was selected from 134 strains of marine microorganism have good antifungal activities.Through bioassay-guided purification,one antifungal compound JWH-09-A was separated from fermentation broth via silica gel column chromatography,thin layer chromatography and pre-HPLC.The compound was identified as 1-phenanthrenecarboxylicacid,1,2,3,4,4a,9,10,10a-octahydro-1,4a-dimethyl-7-(1-methylethyl) by ESI-MS,EI-MS,1H NMR,13C NMR and 2D NMR spectra analysis,which was nam... |
| |
| Keywords: | marine actinomycete JWH-09 fungicidal activity isolation and purification dehydroabietic acid |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
