Determination of Sinapic Acid Derivatives in Canola Extracts Using High-Performance Liquid Chromatography |
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Authors: | Rabie Khattab Michael Eskin Michel Aliani Usha Thiyam |
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Affiliation: | (1) Department of Human Nutritional Sciences, University of Manitoba, 196 Innovation Drive, Winnipeg, MB, R3T 6C5, Canada;(2) Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, 196 Innovation Drive, Winnipeg, MB, R3T 6C5, Canada; |
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Abstract: | A high-performance liquid chromatographic (HPLC) method with diode array detection (DAD) was used to determine the total phenolics,
including sinapic acid derivatives in canola. Ten Western Canadian canola seeds, six other commodity canola seeds, their corresponding
press cakes and meals were analyzed. Seeds of European 00 rapeseed and Brassica Juncea (Indian mustard) were included for comparison. Phenolic compounds were separated using a gradient elution system of water–methanol-ο-phosphoric acid solution with a flow rate of 0.8 ml/min. In addition to sinapine (SP) and sinapic acid (SA), sinapoyl glucose
(SG) is reported in the methanolic extracts. The detection and quantification limits of these compounds were 0.20–0.40 and
0.50–0.80 μg/ml, respectively with recovery values over 98.0%. The content of total phenolics, SP, SA and SG in canola extracts
ranged from 9.16 to 16.13, 6.39 to 12.28, 0.11 to 0.59 and 1.36 to 7.50 mg/g, respectively with significant differences among
varieties. |
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