| 阳极氧化纳米膜上酶固定技术的验证与应用 |
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| 引用本文： | 楼姝珏.阳极氧化纳米膜上酶固定技术的验证与应用[J].广州化工,2014(24):100-102. |
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| 作者姓名： | 楼姝珏 |
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| 作者单位： | 浙江经贸职业技术学院,浙江 杭州,310018 |
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| 摘 要： | 对阳极氧化纳米膜烷硅化和活化的表面处理后，通过与赖氨酸进行取代反应达到固定酶的目的。以蔗糖和葡萄糖为反应物，在被固定的转化酵素-葡萄糖氧化酶-辣根过氧化物酶的催化作用下，利用实时检测510 nm波长下醌亚胺的光吸收，验证了上述酶固定法在生物反应器应用中的可行性。
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| 关 键 词： | 阳极氧化纳米膜 酶固定 生物反应器 |
Enzyme Immobilization and Analysis in Invertase-Glucosidase (GO)-Horse Radish Peroxidise (HRP) System |
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| LOU Shu-jue.Enzyme Immobilization and Analysis in Invertase-Glucosidase (GO)-Horse Radish Peroxidise (HRP) System[J].GuangZhou Chemical Industry and Technology,2014(24):100-102. |
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| Authors: | LOU Shu-jue |
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| Affiliation: | LOU Shu-jue;Zhejiang Economic & Trade Polytechnic; |
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| Abstract: | Inertase, Glucosidase （ GO ） and horse radish peroxidase （ HRP ） were immobilized on nanoporous anodized aluminum oxide membranes respectively, and then were settled in a closed circulation bioreactor system. The formation of quinoneimine dye was monitored under the absorption at 510 nm. A linear relationship between absorption and time was observed. As a result, the immobilization technique and its feasibility in an invertase-GO-HRP bioreactor system were validated. |
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| Keywords: | nanoporous enzyme immobilization bioreactor |
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