| 化学合成胸腺素α1反相色谱分离影响因素的研究 |
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| 引用本文： | 赵玉兰,范业梅,阎金川,廖杰.化学合成胸腺素α1反相色谱分离影响因素的研究[J].现代仪器,2009,15(6). |
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| 作者姓名： | 赵玉兰 范业梅 阎金川 廖杰 |
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| 作者单位： | 解放军总医院医学实验测试中心,北京,100853 |
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| 摘 要： | 胸腺素α1 (Tα1)是一种具有免疫活性的多肽,用固相多肽合成法合成Tα1及肽链长度不等的系列多肽。优化这些多肽的反相分离方法,探讨流动相缓冲体系、pH、梯度洗脱时间、以及柱温对分离的影响。
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| 关 键 词： | 胸腺素 α(Tα1) 反相色谱 |
Study on the reversed phase chromatographic separation of synthesized thymosin-α1 |
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| Zhao Yulan,Fan Yemei,Yan Jinchuan,Liao Jie.Study on the reversed phase chromatographic separation of synthesized thymosin-α1[J].Modern Instruments,2009,15(6). |
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| Authors: | Zhao Yulan Fan Yemei Yan Jinchuan Liao Jie |
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| Abstract: | Thymosin alpha 1 (Tα1) is a peptide that has been evaluated for its immunomodulatory activities.Tα1 and its homologous compounds were synthesized by solid phase peptide synthesis.A high performance reversed phase chromatographic method was studied for the separation of these peptides.Several buffer systems were evaluated for optimizing the selectivity,the effect of gradient eluent time,the mobile phase pH and column tem- perature on the separation was investigated. |
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| Keywords: | Thymosin alpha 1 (Tα1) Reversed phase chromatography |
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