| 用于酶热传感器的酶标甲胺磷农药的制备 |
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| 引用本文： | Nguyen Van-luc,于劲松,徐斐,华泽钊.用于酶热传感器的酶标甲胺磷农药的制备[J].食品科学,2012,33(6):54-58. |
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| 作者姓名： | Nguyen Van-luc 于劲松 徐斐 华泽钊 |
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| 作者单位： | 上海理工大学食品安全研究所 |
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| 基金项目： | 上海市科委重点项目(10391901500);上海市科技兴农重点攻关项目(2006第7-4号) |
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| 摘 要： | 采用碳二亚胺(EDC)法进行酶标甲胺磷的制备研究。考察甲胺磷和丁二酸酐比例、反应时间对甲胺磷衍生物合成的影响，最终确定二者的最适物质的量比为1:1.5，最适反应时间为15h；甲胺磷衍生物与葡萄糖氧化酶比例对酶标甲胺磷偶联率及酶活的影响实验结果显示，随着甲胺磷衍生物与葡萄糖氧化酶二者比例的增加，酶标农药的偶联率增加，但同时也造成了酶活的降低。考虑到酶标农药还应具备足够的酯酶抑制活性，甲胺磷衍生物与葡萄糖氧化酶二者物质的量比为10:1时制得的酶标农药为最优。
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| 关 键 词： | 甲胺磷衍生物 葡萄糖氧化酶(GOD) 酶标农药 |
Preparation of Enzyme-Labeled Methamidophos for Application in Enzyme Thermal Sensor |
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| Nguyen Van-luc,YU Jin-song,XU Fei,HUA Ze-zhao.Preparation of Enzyme-Labeled Methamidophos for Application in Enzyme Thermal Sensor[J].Food Science,2012,33(6):54-58. |
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| Authors: | Nguyen Van-luc YU Jin-song XU Fei HUA Ze-zhao |
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| Affiliation: | (Institute of Food Safety, University of Shanghai for Science and Technology, Shanghai 200093, China) |
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| Abstract: | The preparation of enzyme-labeled methamidophos was carried out by carbodiimide(EDC) method.Based on the effect on the remaining amount of methamidophos,the optimal ratio of methamidophos to succinic anhydride was determined to be 1:1.5 and the optimal reaction time 15 h.With increasing ratio between methamidophos derivatives and glucose oxidase,the coupling rate of enzyme-labeled methamidophos increased,while the enzyme activity decreased.Considering that enzyme-labeled pesticides need sufficient esterase inhibitory activity,the ratio of methamidophos derivatives to glucose oxidase was determined as 10:1. |
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| Keywords: | methamidophos derivative glucose oxidase(GOD) enzyme-labeled pesticides |
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