以6-姜酚肟为内标测定生姜及其制品中6-姜酚的含量

建立了一种以6-姜酚肟代替6-姜酚为标样,采用高效液相色谱法,精确测定生姜及其制品中6-姜酚的方法。采用的色谱柱为Diamonsil C_(18)柱,流动相为乙腈-水系统梯度洗脱,流速1mL/min,检测波长280nm。结果表明:6-姜酚在10.55～2700βg/mL、6-姜酚肟在10.43～2670μg/mL时峰面积与浓度呈良好的线性关系,相关系数分别为0.9983和0.9998。6-姜酚的检测限为2.12μg/mL,6-姜酚肟的检测限为2.09μg/mL。6-姜酚对6-姜酚肟的相对校正因子为1.285,相对保留时间为1.728。6-姜酚肟的加标回收率为96.3%～102.2%,相对标准偏差<2.4%。用内标法和外标法测得的结果无显著差异,测得鲜姜、干姜和姜酒中6-姜酚含量分别为1.86、3.04、0.115 mg/g。用6-姜酚肟做内标测定6-姜酚的方法准确可靠,可用于生姜及其制品中6-姜酚的测定。

Determination of 6-gingerol in Ginger and Its Produces by Using 6-gingerol Oxime as the Internal Standard

To detect the 6—gingerol in ginger and its products,a method with high performance liquid chromatography(HPLC)was investigated with 6—gingerol oxime as the internal standard substance.HPLC determination was performed on a Diamonsil C18 column and detected at 280 nm.The mobile phase consisted of acetonitrile and water,the flow rate was maintained at 1 mL/min.The method was proved to be linear in the ranges of 10.55～2700μg/mL(r=0.9983)for 6—gingerol and 10.43～2670μg/mL(r=0.9998)for its oxime.The limits of detection were 2.12μg/mL for 6—gingerol and 2.09μg/mL for its oxime.The relative correcting factor and relative retention time of 6—gingerol oxime and 6—gingerol were 1.285 and 1.728.The recoveries were in the range of 96.3%～102.2% and relative standard deviations were less than 2.4% for the oxime.There was no distinct difference between the external standard method and the internal standard method.The contents of 6—gingerol in fresh ginger,dried ginger,ginger wine were measured as 1.86,3. 04,0.115 mg/g respectively.This method is simple,fast,accurate and convenient.