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| 麦胚抗氧化肽水解用酶的筛选研究 | |
| 引用本文： | 程云辉,文新华,王璋. 麦胚抗氧化肽水解用酶的筛选研究[J]. 中国粮油学报, 2007, 22(3): 29-33 |
| 作者姓名： | 程云辉 文新华 王璋 |
| 作者单位： | 1. 长沙理工大学生物与食品学院,长沙,410076 2. 湖南商学院,长沙,410000 3. 江南大学食品学院,无锡,214036 |
| 摘 要： | 研究了中性蛋白酶AS1.398、Neutrase及碱性蛋白酶Alcalase、Proleather FG-F、Protease S水解麦胚蛋白的进程特性,考察了5种蛋白酶水解物的体外抗氧化活性。结果表明,碱性蛋白酶Proleather FG-F是制备麦胚抗氧化肽的最适水解酶,其酶解物清除超氧阴离子自由基(O2-.)的能力最强,IC50为1.33mg/mL;其酶解物中相对分子质量小于1000的组分所占比例最高,达到68.28%。 |
| 关 键 词： | 麦胚蛋白 抗氧化肽 蛋白酶 水解 |
| 收稿时间： | 2007-01-27 |
| 修稿时间： | 2007-01-27 |
Selection of Protease for Preparing Antioxidant Peptides from Wheat Germ Protein |
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| Cheng Yunhui,Wen Xinhua,Wang Zhang. Selection of Protease for Preparing Antioxidant Peptides from Wheat Germ Protein[J]. Journal of the Chinese Cereals and Oils Association, 2007, 22(3): 29-33 | |
| Authors: | Cheng Yunhui Wen Xinhua Wang Zhang |
| Abstract: | Five proteases,AS1.398,Neutrase,Alcalase,Proleather FG-F,and Protease S were investigated to hydrolyze wheat germ protein respectively for the purpose of selecting the best protease to prepare antioxidant peptides from the wheat germ protein.Results show that Proleather FG-F is the best protease;the wheat germ protein hydrolysates prepared by Proleather FG-F exhibits the strongest scavenging activity against superoxide radical with IC50 being 1.33 mg/mL and have the highest content of component with relative molecular weight under 1 000,up to 68.28%. |
| Keywords: | wheat germ protein antioxidant peptide protease hydrolyze |
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