响应曲面优化超声辅助提取山茱萸籽油工艺及其成分分析

研究以正己烷为溶剂超声辅助提取山茱萸籽油的工艺，并对其成分进行分析。采用响应曲面优化法(RSM)优化山茱萸籽油的提取工艺，确定最佳提取工艺参数为提取温度40℃、提取时间40min、液料比10:1(mL/g)，在此条件下，山茱萸籽油的一次提取率为97.08%(出油率7.90%)。利用GC-MS对山茱萸籽油中脂肪酸成分进行分析，结果表明：山茱萸籽油的主要脂肪酸成分为油酸(61.89%)、硬脂酸(19.99%)、棕榈酸(7.63%)和亚油酸(6.33%)等，不饱和脂肪酸占脂肪酸总量的69.45%；采用高效液相色谱法测定，山茱萸籽油中VE含量为76.0mg/100g。

Optimization of Ultrasound-Assisted Extraction of Cornus officinalis Seed Oil by Response Surface Methodology and Chemical Composition Analysis

Response surface methdology was employed to optimize the ultrasound-assisted extraction of Cornus officinalis seed oil.Meanwhile,its chemical composition was analyzed by GC-MS.The optimal extraction conditions were determined as 40 ℃ of extracion temperatrure,40 min of extraction time,and 10:1 of liquid-to-solid raio(mL/g).Under these conditions,the extraction yield of Cornus officinalis seed oil based on the dry weight of Cornus officinalis kernels was 7.90%,accounting for 97.08% of that obtained from Soxhlet extraction.The predominant fatty acids in Cornus officinalis seed oil were oleic acid(61.89%),stearic acid(19.99%),palmitic acid(7.63%),linoleic acids(6.33%),and so on.The percentage of unsaturated fatty acids relative to total fatty acids was 69.45%.In addition,the vitamin E content in Cornus officinalis seed oil,as determined by HPLC,was 76.0 mg/100 g.