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Solution properties of polyelectrolytes. II. Comparison of the effects of both polyion and eluent salt concentrations on the calibrations in aqueous exclusion chromatography
Authors:Concepci  n Abad,Lorenzo Braco,Vicente Soria,Rosa Garcí  a,Agustí  n Campos
Affiliation:Concepción Abad,Lorenzo Braco,Vicente Soria,Rosa García,Agustín Campos
Abstract:The elution behaviour of charged water-soluble polymers (sodium polystyrene sulphonate) in both salt-free and NaCl-containing aqueous mobile phases has been investigated by size-exclusion chromatography. A comparative analysis has been established between the results as a function of polyion and salt concentrations, which demonstrates that the chromatographic behaviour is similar in both cases. The variation of the slopes of the different concentration-dependent calibration curves is discussed in terms of polyelectrolyte conformational changes. Finally, a semi-empirical model has been developed to predict the dependence of the elution volume in either polyion or salt concentration, and its validity has been checked in a range from very dilute to moderate concentrations.
Keywords:Polyelectrolytes  size-exclusion chromatography  salt-free water  effect of ionic strength  semi-empirical model
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