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蚕丝木瓜蛋白酶脱胶工艺条件探讨
引用本文:邓一民,张高军,敬凌霄,温少美,王平. 蚕丝木瓜蛋白酶脱胶工艺条件探讨[J]. 丝绸, 2009, 0(8)
作者姓名:邓一民  张高军  敬凌霄  温少美  王平
作者单位:西南大学,纺织服装学院,重庆,400715
基金项目:重庆市教委科学技术研究项目 
摘    要:研究脱胶条件对蚕丝木瓜蛋白酶脱胶效果的影响.由单因子实验选出合适的酶质量浓度、温度、时间、pH值范围,用正交实验优化脱胶条件.结果表明.木瓜蛋白酶能有效地脱除丝胶:脱胶率和丝的强力损失率,随着酶质量浓度增加、pH值及温度的升高和脱胶时间的延长而上升;正交实验证实酶质量浓度是影响脱胶率的丰要因子,酶脱胶时间是强力损失的主要影响因素;综合考虑脱胶率和强力损失率,确定生丝木瓜蛋白酶脱胶的2个较好工艺条件为:一是酶质量浓度3 g/L、pH6、温度85℃、时间60 min;二是酶质量浓度3 g/L、pH8、温度65℃、时间90 min.

关 键 词:木瓜蛋白酶  蚕丝  脱胶率  强力损失率  脱胶条件

Investigating the Process Conditions of Mulberry Silk Degumming with Papain
DENG Yi-min,ZHANG Gao-jun,JING Ling-xiao,WEN Shao-mei,WANG Ping. Investigating the Process Conditions of Mulberry Silk Degumming with Papain[J]. Silk Monthly, 2009, 0(8)
Authors:DENG Yi-min  ZHANG Gao-jun  JING Ling-xiao  WEN Shao-mei  WANG Ping
Affiliation:College of Textile and Clothing;Southwest University;Chongqing 400715;China
Abstract:This study investigated whether the degumming conditions had effect on mulberry silk degumming with papain.It used single-factor experiment to pick up the proper enzyme quality-concentration,temperature,time,pH value range,and then obtained optimized degumming conditions by orthogonal experiment.The results indicated that papain can effectively remove sericin.The results of the single-factor experiments showed the degumming loss rates and silk degumming strength loss rate are increasing with the enzyme conc...
Keywords:Papain  Mulberry silk  Degumming loss rate  Breaking strength loss rate  Degumming conditions
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