Analysis of bacteria in aquatic environments using sedimentation field-flow fractionation: (II) physical characterization of cells |
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| Affiliation: | 1. Division of Pharmacoengineering and Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina-Chapel Hill, 27519, Chapel Hill, NC, USA;2. UNC/NCSU Joint Department of Biomedical Engineering, University of North Carolina-Chapel Hill, 27519, Chapel Hill, NC, USA;3. Mapp Biopharmaceutical Inc, 92121, San Diego, CA, USA;4. Department of Microbiology and Immunology, University of Maryland School of Medicine, 21201, Baltimore, MD, USA;5. Institute for Genome Sciences, University of Maryland School of Medicine, 21201, Baltimore, MD, USA;1. Department of Biophysics, Johns Hopkins University, Baltimore, Maryland, USA;2. UNC/NCSU Joint Department of Biomedical Engineering, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina, USA;3. Division of Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina—Chapel Hill, Chapel Hill, North Carolina, USA;4. ReProtect, Baltimore, Maryland, USA;1. The Hakubi Center for Advanced Research, Kyoto University, Kyoto 606-8501, Japan;2. Research Institute for Mathematical Sciences, Kyoto University, Kyoto 606-8502, Japan;3. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Czech Republic;4. Wolfson Centre for Mathematical Biology, Mathematical Institute, University of Oxford, Oxford OX2 6GG, UK;1. Institute for the Reproduction of Farm Animals Schönow Inc., Bernauer Allee 10, D-16321 Bernau, Germany;2. University of Applied Science, Wilhelminenhofstraße 75A, D-12459, Berlin, Germany;1. Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA;2. Noble Equine Veterinary Service, Purcell, Oklahoma, USA;3. Katy, Texas, USA;4. Houston, Texas, USA;5. Department of Animal and Dairy Science, Mississippi State University, Starkville, Mississippi, USA;1. Institute for the Reproduction of Farm Animals Schönow e.V. (IFN), Bernauer Allee 10, D-16321 Bernau, Germany;2. Unit for Reproductive Medicine of Clinics/Clinic for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, Hannover, Germany |
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| Abstract: | This paper describes the application of sedimentation field-flow fractionation (SdFFF) for the physical characterization of bacteria cells found in natural aquatic environments. This technique separates bacteria on the basis of the buoyant mass of the cells and fractions can be collected for determination of cell numbers and volume. This enables the cell density and size distribution of the sample to be calculated. SdFFF is also capable of generating very detailed cell size and specific gravity distributions which may be useful data in assessing the bacterial growth status of natural waters. Data for samples from seawater, two lakes and a sewer drain are given. The specific gravity of cells varies both within and between samples and falls in the range 1.03–1.2 g cm−3. This contrasts with previous work which shows little variation within a given cultured bacteria sample and indicates that natural populations contain a range of different bacteria types. |
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