Type I collagen-templated assembly of silver nanoparticles and their application in surface-enhanced Raman scattering |
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Authors: | Sun Yujing Wei Gang Song Yonghai Wang Li Sun Lanlan Guo Cunlan Yang Tao Li Zhuang |
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Affiliation: | State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun 130022, People's Republic of China. |
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Abstract: | Silver nanoparticles (Ag NPs) are one of the active substrates that are employed extensively in surface-enhanced Raman scattering (SERS), and aggregations of Ag NPs play an important role in enhancing the Raman signals. In this paper, we fabricated two kinds of SERS-active substrates utilizing the electrostatic adsorption and superior assembly properties of type I collagen. These were collagen-Ag NP aggregation films and nanoporous Ag films. Two probe molecules, 4-aminothiophenol (4-ATP) and methylene blue (MB), were studied on these substrates. These substrates showed reproducible SERS intensities with relative standard deviations (RSDs) of 8-10% and 11-14%, respectively, while the RSDs of the traditional thick Ag films were 12-28%. Also, the intensities for the 4-ATP spectrum on the collagen-templated nanoporous Ag film were approximately one order higher than those on the DNA-templated Ag?film. |
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