A dose-controlled system for air-liquid interface cell exposure and application to zinc oxide nanoparticles |
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| Authors: | Anke Gabriele Lenz Erwin Karg Bernd Lentner Vlad Dittrich Christina Brandenberger Barbara Rothen-Rutishauser Holger Schulz George A Ferron Otmar Schmid |
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| Affiliation: | 1. Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Lung Biology and Disease, Ingolstaedter Landstrasse 1, D-85758, Neuherberg, Germany
2. Division of Histology, University of Bern, Institute of Anatomy, Baltzerstrasse 2, CH-3000, Bern 9, Switzerland
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| Abstract: | Background Engineered nanoparticles are becoming increasingly ubiquitous and their toxicological effects on human health, as well as on the ecosystem, have become a concern. Since initial contact with nanoparticles occurs at the epithelium in the lungs (or skin, or eyes), in vitro cell studies with nanoparticles require dose-controlled systems for delivery of nanoparticles to epithelial cells cultured at the air-liquid interface. |
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