Carbohydrate polymers of chirata (Swertia chirata) leaves: Structural features, in vitro anti-oxidant activity and fluorescence quenching study |
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Authors: | Debjani Ghosh Shruti S Bandyopadhyay Udipta R Chatterjee Peter Capek Bimalendu Ray |
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Affiliation: | 1. Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Burdwan, West Bengal, 713 104, India 2. Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dubravska cesta 9, SK-845 38, Bratislava, Slovakia
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Abstract: | Chirata (Swertia chirata) is widely used in Indian folk medicine for the prevention of various disorders. Herein, we analyzed the water-extracted
carbohydrate polymer (WECP) of this herb using chemical, chromatographic, and spectroscopic methods. The anti-oxidant capacity
of this fraction was studied by ferric reducing anti-oxidant power (FRAP) and DPPH radical assays. Effect of WECP on bovine
serum albumin spectrum (BSA) was determined using excitation wavelength of 282 nm. Anion exchange chromatography of WECP yielded
5 fractions (F1–F5) with different chemical compositions. The major fraction (F5) was homogeneous, had an apparent molecular
mass of 4.5 kDa, and contains both carbohydrates (57%) and phenolics (34%). The anti-oxidant capacities of WECP and F5 were
comparable to standard anti-oxidants. Notably, activities of the carbohydrate polymers (F1–F5) correlate with their phenolics
content. Fluorescence quenching measurement suggests that F5 can form complex with BSA and the value of the binding constant
is K=6.28×105/M. |
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