IDENTIFICATION OF GOAT MEAT USING HIGHLY SPECIES‐SPECIFIC POLYMERASE CHAIN REACTION |
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| Authors: | NAGAPPA S. KARABASANAVAR S.P. SINGH UMAPATHI V. DEEPAK KUMAR SUNIL N. SHEBANNAVAR |
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| Affiliation: | 1. Department of Veterinary Public Health, Mumbai Veterinary College, Mumbai‐400 012, Maharashtra 412 801, India;2. Department of Veterinary Public Health, College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India;3. Indian Council of Agricultural Research, Palampur, Himachal Pradesh, India;4. Division Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India;5. Gennova Biopharmaceutical Pvt. Ltd, Pune, India |
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| Abstract: | ABSTRACT A highly species‐specific polymerase chain reaction (PCR) assay was developed for the authentic identification of goat. A product of 436 bp was amplified using newly designed primers against mitochondrial D‐loop region. The possibility of cross‐amplification was ruled out by considering as many as 25 other animal species. Suitability of the developed goat species‐specific PCR assay was confirmed for in raw, cooked (60, 80 and 100C for 30 min) and micro‐oven‐processed meat samples (n = 20 each). A sensitivity of 0.1% was established for detection of adulteration and limit of detection of goat DNA was 0.1 pg. This investigation presents a novel PCR assay with its newly designed primers that could be used for the authentic identification of goat species. PRACTICAL APPLICATIONS This work details about a novel diagnostic polymerase chain reaction, which could be used for authentic identification of goat species. This approach could be used for the confirmation of goat tissues in raw, cooked, as well as adulterated samples. The developed technique has also applications in the forensic analysis of wild animal‐related disputes, where this work could solve the problem of goat‐related issues. |
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