| 从RNA酶解液中分离5-尿苷酸 |
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| 引用本文： | 李德莹,丁庆豹. 从RNA酶解液中分离5-尿苷酸[J]. 湖北化工, 2011, 0(12): 73-76 |
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| 作者姓名： | 李德莹 丁庆豹 |
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| 作者单位： | [1]三峡大学化学与生命科学学院,湖北宜昌443002 [2]华东理工大学生物反应器工程国家重点实验室,上海200237 |
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| 摘 要： | 采用2根强酸性阳离子交换柱、1根弱碱性阴离子交换柱和1根强碱性阴离子交换柱进行4柱串联，可以从RNA酶解液中分离得到5-尿苷酸，而不混有其它核苷酸，并对离子交换树脂种类、树脂量、洗脱剂等作了进一步研究。结果表明，采用4柱串联分离5-尿苷酸，其总收率达到92．1％、结晶纯度达到86％以上。
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| 关 键 词： | 5-尿苷酸 酵母RNA 离子交换 |
Separation of Uridine 5-Monophosphate from the Enzymatic Degradation Solution of RNA |
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| LI De-ying,DING Qing-bao. Separation of Uridine 5-Monophosphate from the Enzymatic Degradation Solution of RNA[J]. Hubei Chemical Industry, 2011, 0(12): 73-76 |
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| Authors: | LI De-ying DING Qing-bao |
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| Affiliation: | 1. College of Chemistry and Life Science, Three Gorges University ,Yichang 443002, China ; 2. State Key Laboratory of Bioreactor Engineering ,East China University of Science and Technology, Shanghai 200237, China) |
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| Abstract: | Uridine 5-monophosphate（UMP） could be separated from the enzymatic degradation solution of RNA with two strong acid cation exchange columns,one weak base anionic exchange column and one strong al- kali anionic exchange column. UMP Obtained was not contaminated with other three ribonucleotides. The total yield of UMP achieved 92.1% and the purity of UMP crystal reached above 86%. The type of ion exchange res- in,resin amount and eluent were further studied. |
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| Keywords: | uridine 5 -monophosphate i yeast RNA i ion exchange |
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