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Production of nutraceutics from chestnut burs by hydrolytic treatment
Affiliation:1. Departamento de Enxeñería Química, Universidade de Vigo (Campus Ourense), Edificio Politécnico, As Lagoas, 32004 Ourense, Spain;2. Departamento de Química Analítica y Alimentaria, Universidade de Vigo (Campus Ourense), Edificio Politécnico, As Lagoas, 32004 Ourense, Spain;1. Centre for the Research and Technology of Agro-Environmental and Biological Sciences, CITAB, University of Trás-os-Montes and Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real, Portugal;2. Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), Campus Universitario de Espinardo-25, E-30100 Murcia, Spain;1. Programa de Pós-graduação em Ciência e Tecnologia de Alimentos (PPGCTA), Universidade Federal Rural de Rio de Janeiro (UFRRJ), Rodovia BR 465, Km 7, Seropédica, RJ 23890-000, Brazil;2. Laboratório de Engenharia e Tecnologia Agroindustrial (LETA), Universidade Federal Fluminense (UFF), Av. dos Trabalhadores, 420, Volta Redonda, RJ 27255-125, Brazil;3. Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa (UFV), 36571-000 Viçosa, MG, Brazil;1. IEM (Institut Européen des Membranes), UMR 5635 (CNRS-ENSCM-UM2), Université Montpellier 2, Place E. Bataillon, F-34095 Montpellier, France;2. Laboratory of Materials Science and Environment, University of Sfax, Faculty of Science of Sfax, Route de la Soukra Km 3.5, BP 1171, Sfax 3000, Tunisia
Abstract:Chestnut (Castanea sativa) burs (CB) were processed by solvent extraction (with ethanol, toluene–ethanol or n-hexane) and/or isothermal autohydrolysis, and the potential as nutraceutics of the solubilized fractions of dietary fiber and antioxidants was evaluated. Among the studied alternatives, the most suited consisted on ethanol extraction, which yielded 21.0 g gallic acid equivalents (GAE)/100 g ethanolic CB extract, with EC50,DPPH = 0.38 g/L. Ethanol-extracted CB were treated with water at 120 °C, and the soluble fraction was mixed with ethanol to precipitate 8.8 g soluble fiber/100 g CB, with a Water Retention Capacity of 2.4 g/g and an oil absorption capacity of 23.7 g/g. The mother liquors from the above precipitation stage were concentrated and extracted with ethyl acetate to yield an organic phase containing soluble phenolics with high antioxidant activity (EC50,DPPH = 0.103 g/L) at a yield of 7 g/100 g CB. When the aqueous phase before the ethyl acetate extraction was treated with sulfuric acid, an additional antioxidant fraction (EC50,DPPH = 0.395 g/L) was obtained. The properties and characteristics of these extracts produced make them good candidates to be used as active ingredients.
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