Methanogenic activity optimization using the response surface methodology,during the anaerobic co-digestion of agriculture and industrial wastes. Microbial community diversity |
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| Affiliation: | 1. Universidad de Sancti Spíritus, Ave. de los Mártires, No 360, CP 60100 Sancti Spíritus, Cuba;2. Universidad de Granma, Carretera de Manzanillo, Km 17 ½, CP 85100 Bayamo, Granma, Cuba;3. Instituto de Ingeniería, Universidad Nacional Autónoma de México, Circuito Escolar S/N Ciudad Universitaria, Delegación Coyoacán, CP 04510 D.F. México, Mexico;4. Facultad de Biología, Universidad de La Habana, Calle 25 e/ I y J, Vedado, CP 10400 La Habana, Cuba;1. Department of Chemical Engineering, University of Barcelona, C/Martí i Franquès, no. 1, 6th floor, 08028 Barcelona, Spain;2. Advanced Water Management Centre, The University of Queensland, St Lucia, QLD 4072, Australia;1. IRTA, GIRO Joint Research Unit IRTA-UPC, Torre Marimon, E-08140, Caldes de Montbui, Barcelona, Spain;2. EnergyLab, Edificio CITEXVI – Local 1, Fonte das Abelleiras, s/n, Campus Universitario de Vigo, E-36310 Vigo, Spain;3. GIRO Joint Research Unit IRTA-UPC, Department of Agrifood Engineering and Biotechnology, Universitat Politècnica de Catalunya – BarcelonaTECH, Parc Mediterrani de la Tecnologia, Building D-4, E-08860, Castelldefels, Barcelona, Spain;1. Shandong Industrial Engineering Laboratory of Biogas Production & Utilization, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong Province 266101, PR China;2. University of Chinese Academy of Sciences, Beijing 100049, PR China;1. Bioresources Development Centre, National Biotechnol. Development Agency, Ogbomoso, Nigeria;2. Biochemical Engineering Lab., Department of Chemical Engineering, Obafemi Awolowo University, Ile-Ife, Nigeria;3. Department of Biological Sciences, Florida Agricultural and Mechanical University, Tallahassee, FL, 32307, USA;4. Department of Microbiology, Bowen University, Iwo, Oyo State, Nigeria |
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| Abstract: | The anaerobic co-digestion of manure, agriculture and industrial wastes for methane production depends on the nutritional condition to develop the microbial community. The effect of each substrate concentrations, as well as their interactive effects on specific methanogenic activity and microbial community diversity were investigated in this work. A central composite design and the response surface methodology were applied for designing the anaerobic co-digestion batch test at 35 and 55 °C. It was analyzed the anaerobic sludge by specific methanogenic activity (SMA) and using molecular techniques (terminal restriction fragment length polymorphism, TRFLP). The results showed a significant interaction among the substrates and an enhancement of the methane production and SMA response caused by the three components. Rice straw had lower influence on SMA than clay residues, due to the mineral content and the beneficial ammonia nitrogen adsorbent properties of the latter. The optimum condition for mesophilic and thermophilic anaerobic co-digestion of pig manure, rice straw and clay mixture allowed SMA values of 1.31 and 1.38 gCH4-COD/gVSSd−1, respectively. The TRFLP analysis showed the effect of rice straw and clay addition on microbial community diversity at both temperatures. The acetotrophic methanogens belonging to the order Methanosarcinales (genera Methanosarcina and Methanosaeta) dominated in mesophilic condition, whereas at thermophilic conditions dominated Methanomicrobiales and Methanobacteriales order. The optimization allowed identifying the substrate interaction effects in a concentration range with a reduced number of experiments. Besides, the model validation proved to be useful for defining optimal combination of wastes in anaerobic system. |
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| Keywords: | Clay Pig manure Optimization Rice straw Specific methanogenic activity Microbial community |
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