| 双酶法制备玉米肽酶解条件研究 |
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| 引用本文： | 李艳丽,丛建民,陈光.双酶法制备玉米肽酶解条件研究[J].食品科学,2010,31(1):145-148. |
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| 作者姓名： | 李艳丽 丛建民 陈光 |
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| 作者单位： | 1.吉林农业大学生命科学学院 2.白城师范学院生物系 |
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| 基金项目： | 吉林省科技厅资助项目(20020211) |
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| 摘 要： | 利用双酶法水解玉米蛋白粉制备玉米肽，探讨各因素对水解度(DH)的影响，旨在确定玉米肽制备的最佳酶解条件。结果表明：最佳酶解条件为温度55℃、底物质量浓度5g/100mL、pH8.5，按4% 的酶与底物比加碱性蛋白酶水解1.5h 后，调整pH 值至7.5，之后再按3.5% 的酶与底物比加木瓜蛋白酶水解1.5h。在此条件下，水解液中DH 为80.97%。与单酶法相比，DH 明显提高。
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| 关 键 词： | 碱性蛋白酶 木瓜蛋白酶 双酶法 玉米肽 水解条件 |
| 收稿时间： | 2009-03-25 |
Double Enzyme Hydrolysis for Preparation of Corn Peptides |
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| LI Yan-li,CONG Jian-min,CHEN Guang.Double Enzyme Hydrolysis for Preparation of Corn Peptides[J].Food Science,2010,31(1):145-148. |
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| Authors: | LI Yan-li CONG Jian-min CHEN Guang |
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| Affiliation: | (1. College of Life Science, Jilin Agricultural University, Changchun 130118, China；
2. Department of Biology, Baicheng Normal College, Baicheng 137000, China |
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| Abstract: | Double enzyme hydrolysis was used to prepare peptides from corn gluten meal. In order to maximize degree of hydrolysis (DH), crucial hydrolysis parameters were optimized. Results showed that a maximum DH of 80.97% was obtained by optimized sequential hydrolysis for 1.5 h using protease alkaline at 55 ℃ and pH 8.5 with 5 g/100mL substrate concentration and 4% enzyme dose followed by 1.5 h using papain at pH 7.5 with 3.5% enzyme dose, which was significantly higher that obtained by single enzyme hydrolysis. |
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| Keywords: | alkaline protease papain double enzyme hydrolysis corn peptides hydrolysis conditions |
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