| 血管活性肠肽的固相合成及其体外抗菌活性研究 |
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| 引用本文： | 徐春兰,尚晓娅,牛卫宁,钦传光. 血管活性肠肽的固相合成及其体外抗菌活性研究[J]. 湖北化工, 2012, 0(5): 12-15 |
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| 作者姓名： | 徐春兰 尚晓娅 牛卫宁 钦传光 |
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| 作者单位： | 西北工业大学生命学院空间生物实验模拟技术国防重点实验室,陕西西安710072 |
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| 基金项目： | 国家自然科学基金资助项目（31001012）,西北工业大学基础科学研究基金资助项目（JC201278） |
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| 摘 要： | 采用Na-芴甲氧羰基（FMOC）作为α-氨基的保护基,以逐个延伸的固相合成法合成了血管活性肠肽,以最小抑菌浓度（MIC）评价其体外抗菌活性。高效液相色谱和质谱分析表明,所合成的血管活性肠肽的纯度为95.2%,相对分子质量为3326.5。血管活性肠肽对几种革兰氏阳性菌和革兰氏阴性菌有不同程度的抑制活性,其中对E.coli和P.aeruginosa的抑制效果最好。
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| 关 键 词： | 血管活性肠肽 固相合成 抗菌活性 |
Study on Solid Synthesis and Antimicrobial Activity in Vitro of Vasoactive Intestinal Peptide |
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| XU Chun-lan,SHANG Xiao-ya,NIU Wei-ning,QIN Chuan-guang. Study on Solid Synthesis and Antimicrobial Activity in Vitro of Vasoactive Intestinal Peptide[J]. Hubei Chemical Industry, 2012, 0(5): 12-15 |
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| Authors: | XU Chun-lan SHANG Xiao-ya NIU Wei-ning QIN Chuan-guang |
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| Affiliation: | (Key Laboratory for Space Bioscience Biotechnology ,Faculty of Life Sciences, Northwestern Polytechnical University, Xian 710072, China) |
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| Abstract: | Vasoactive intestinal peptide（VIP） was synthesized by FMOC solid phase synthesis method andits antimicrobial activity in vitro was investigated by the determination of minimal inhibitory concentration （MIC）. The HPLC and MS results showed that VIP was synthesized successfully with purity of 95.2% and Mw of 3326.5. VIP Displayed variable degrees of antimicrobial activity against several pathogens tested, espe- cially for E. coli and P. aeruginosa. |
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| Keywords: | vasoactive intestinal peptide（VIP） solid synthesis antimicrobial activity |
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