| 黄芩毛状根中总黄酮的提取工艺 |
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| 引用本文： | 施春阳,齐香君,钱卫东,徐方稳,郝红梅. 黄芩毛状根中总黄酮的提取工艺[J]. 陕西科技大学学报, 2013, 0(2): 105-108 |
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| 作者姓名： | 施春阳 齐香君 钱卫东 徐方稳 郝红梅 |
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| 作者单位： | 陕西科技大学生命科学与工程学院;陕西三正药业有限公司;陕西康惠制药股份有限公司 |
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| 基金项目： | 国家自然科学基金青年项目(31100040);陕西省教育厅专项科研计划项目(11JK0624) |
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| 摘 要： | 以总黄酮含量为指标，优选了树脂型号并考察了影响黄芩毛状根总黄酮提取效果的相关因素．结果显示，LX-22型大孔吸附树脂的提取效果最好，较优工艺条件为：上样浓度为8．69mg·mL-1、吸附流速为6BV·h-1、上样体积为13BV、树脂床径高比为1：14，洗脱剂为70％乙醇、洗脱流速为6BV·h-1、用量为9BV．在所确定的工艺条件下，该树脂可有效地提取黄芩毛状根中的总黄酮，总黄酮吸附量达113．0mg·mL-1，总黄酮的回收率超过829／6．
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| 关 键 词： | 黄芩毛状根 总黄酮 大孔吸附树脂 |
Extraction process of total flavone from hairy roots of Scutellaria baicalensis Georgi |
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| SHI Chun-yang,QI Xiang-jun,QIAN Wei-dong,XU Fang-wen,HAO Hong-mei. Extraction process of total flavone from hairy roots of Scutellaria baicalensis Georgi[J]. Journal of Shaanxi University of Science & Technology(Natural Science Edition), 2013, 0(2): 105-108 |
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| Authors: | SHI Chun-yang QI Xiang-jun QIAN Wei-dong XU Fang-wen HAO Hong-mei |
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| Affiliation: | 1.College of Life Science and Engineering,Shaanxi University of Science & Technology,Xi′an 710021,China;2.Shaanxi Sanzheng Pharmaceutical Co.,Ltd.,Xianyang 712000,China;3.Shaanxi Kanghui Pharmaceutical Co.,Ltd.,Xianyang 712000,China) |
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| Abstract: | Using the content of total flavone as the standard, the optimum macroporous resins were selected and the factors of total flavonets extraction were investigated. LX 22 macro- porous resin was the suitable one, and total flavone was extracted under the optimum condi- tions of a sample concentration of 8.69 mg . mL-1 , a adsorption speed of 6 BV . h-1 ,a diameter-high ratio of 1 ： 14, a sample volume of 13 BV,a elution reagent of 70~/oo alcohol, a e- lution speed of 6 BV . h -1, a elution volume of 9 BV. The LX-22 macroporous resin can be used to extract total flavone from the hairy roots of Scutellaria baicalensis Georgi,the ad- sorption capacity of total flavone was 113. 0 mg . mL-1 and the returns ratio was above 82%. |
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| Keywords: | hairy roots of Scutellaria baicalensis Georgi total flavone macroporous resin |
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