| 重金属铅酶联免疫检测方法的研究 |
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| 引用本文： | 杨依锦,王俊平,王津,方淑兵,王硕. 重金属铅酶联免疫检测方法的研究[J]. 食品与机械, 2012, 28(3): 80-83 |
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| 作者姓名： | 杨依锦 王俊平 王津 方淑兵 王硕 |
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| 作者单位： | 食品营养与安全教育部重点实验室,天津300457;天津科技大学,天津300457 |
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| 摘 要： | 制备铅离子多克隆抗体,并建立间接竞争ELISA检测方法。利用双功能螯合剂ITCBE将Pb2+与载体蛋白KLH和BSA偶联在一起,制备免疫抗原与检测抗原;通过免疫新西兰大耳白免成功制备铅多克隆抗体,建立间接竞争ELISA检测方法,并与ICP-MS检测结果进行比较。标准曲线IC50为3.48ng/mL,IC15为0.32ng/mL,与ICP-MS法检测结果的总符合率超过97%。
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| 关 键 词： | 铅 多克隆抗体 间接竞争ELISA |
Development of enzyme-linked immunosorbent assay for heavy metal lead |
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| YANG Yi-jin , WANG Jun-ping , WANG Jin , FANG Shu-bing , WANG Shuo. Development of enzyme-linked immunosorbent assay for heavy metal lead[J]. Food and Machinery, 2012, 28(3): 80-83 |
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| Authors: | YANG Yi-jin WANG Jun-ping WANG Jin FANG Shu-bing WANG Shuo |
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| Affiliation: | 1,2 (1.Key Laboratory of Food Nutrition and Safety,Ministry of Education of China,Tianjin 300457,China;2.Tianjin University of Science and Technology,Tianjin 300457,China) |
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| Abstract: | To prepare polyclonal antibody against heavy metal lead ion and develop an indirect competitive ELISA method.Lead was coupled to vector proteins keyhole limpet hemocyanin(KLH)and bovine serum albumin(BSA)via a bifunctional chelating agent ITCBE respectively,Pb(Ⅱ)-ITCBE-KLH was used as immunogen and Pb(Ⅱ)-ITCBE-BSA was used as coating antigen.The anti-Pb antibody was obtained after immunized to New Zealand white rabbits and an indirect competitive ELISA method was developed,by which the determination result was compared with that by ICP-MS method.The standard curve was developed with IC50of 3.48 ng/mL and IC15of 0.32 ng/mL,the total coincidence rate of the developed ELISA method and ICP-MS method was more than 97%. |
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| Keywords: | heavy metal lead polyclonal antibody ic-ELISA |
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