| 燕麦蛋白的碱法提取工艺及其提纯研究 |
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| 引用本文： | 张杰,李洋,韩小贤,何义萍,赵亚娟,郑学玲. 燕麦蛋白的碱法提取工艺及其提纯研究[J]. 食品与机械, 2012, 28(4): 121-124 |
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| 作者姓名： | 张杰 李洋 韩小贤 何义萍 赵亚娟 郑学玲 |
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| 作者单位： | 河南工业大学粮油食品学院,河南郑州,450001 |
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| 基金项目： | 国家自然科学基金项目，河南工业大学2010年研究生科技创新基金项目 |
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| 摘 要： | 为提高燕麦蛋白的提取率,采用正交试验对碱提酸沉法提取燕麦蛋白的工艺进行优化,确定其最佳提取工艺条件为料液比1∶13(m∶V),浸提液pH值10.5,浸提温度45℃,搅拌时间1.7h。该条件下燕麦蛋白提取率可达68.46%。经石油醚提纯后燕麦蛋白纯度可达86.35%,有效提高了燕麦蛋白的提取率。
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| 关 键 词： | 燕麦 蛋白 碱提酸沉 纯度 氨基酸分析 |
Research on extraction of oat protein by alkali-extraction and acid precipitation method |
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| ZHANG Jie , LI Yang , HAN Xiao-xian , HE Yi-ping , ZHAO Ya-juan , ZHENG Xue-ling. Research on extraction of oat protein by alkali-extraction and acid precipitation method[J]. Food and Machinery, 2012, 28(4): 121-124 |
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| Authors: | ZHANG Jie LI Yang HAN Xiao-xian HE Yi-ping ZHAO Ya-juan ZHENG Xue-ling |
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| Affiliation: | (Grain College,Henan University of Technology,Zhengzhou,Henan450001,China) |
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| Abstract: | Oat protein has great research value with abundant nutrition.Orthogonal test for optimizing the alkali-extraction and acid precipitation method was carried out in order to enhance the extraction of oat protein.The optimum extraction condition contained : solid-liquid ratio 1∶13,pH 10.5,temperature 45℃and 1.7h.The extraction rate increased effectively to 68.46%under this condition and the purity of oat protein extracted by petroleum ether was 86.35%. |
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| Keywords: | oat protein alkali-extraction and acid precipitation purity amino acid analysis |
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