Energetics and product formation by Saccharomyces cerevisiae grown in anaerobic chemostats under nitrogen limitation |
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Authors: | G Lidén A Persson L Gustafsson C Niklasson |
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Affiliation: | Department of Chemical Reaction Engineering, Chalmers University of Technology, G?teborg, Sweden. |
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Abstract: | Anaerobic fermentation of glucose (20 g/l) by Saccharomyces cerevisiae CBS 8066 was studied in a chemostat (dilution rate = 0.05-0.25 h-1) at different concentrations of the nitrogen source (5.00 g/l or 0.36 g/l ammonium sulphate). The ethanol yield (g ethanol produced/g glucose consumed) was found to be higher and the glycerol yield (g glycerol formed/g glucose consumed) lower during nitrogen limitation than under carbon limitation. The biomass yield on ATP (g dry weight biomass produced/mol ATP consumed), was consequently found to be lower during nitrogen-limited conditions. |
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